Recently, in Prof. Benzhong Tang’s group at the Hong Kong University of Science & Technology, Dr. Xianglong Hu and coworkers provide a strategy for metal-free click bioconjugation based on activated alkynes. The paper entitled “A Simple Approach to Bioconjugation at Diverse Levels: Metal-Free Click Reactions of Activated Alkynes with Native Groups of Biotargets without Prefunctionalization” was recently published in Research (Research 2018 Article ID: 3152870 DOI: 10.1155/2018/3152870) https://spj.sciencemag.org/research/2018/3152870/.
Activated alkynes-based metal-free bioconjugation without prefunctionalization.
The efficient bioconjugation of functional groups/molecules to targeted matrix and bio-related species drives the great development of material science and biomedicine. While the dilemma in traditional bioconjugation, including metal catalyst, uneasy pre-modification, and limited reaction efficiency, has restricted the booming development to some extent.
Recently, in Professor Benzhong Tang’s group at the Hong Kong University of Science & Technology (Homepage: http://tangbz.ust.hk/tbz.html), Dr. Xianglong Hu and coworkers provide a strategy for metal-free click bioconjugation based on activated alkynes. As a proof-of-concept, the abundant native groups including amine, thiol and hydroxyl groups can directly react with activated alkynes without any modification in the absence of metal catalysis. Through this strategy, high-efficient modification and potential functionalization can be achieved for natural polysaccharide, biocompatible polyethylene glycol (PEG), synthetic polymers, cell penetrating peptide, protein, fast whole-cell mapping of living cells, and even quick differentiation and staining of living bacteria, etc. Therefore, current metal-free click bioconjugation strategy based on activated alkynes is promising for the development of quick fluorescence labeling, functional modification of many biotargets, which is favorable for the fabrication of complex biomaterials and future in vivo labeling and detection.
Furthermore, inorganic silicon particles can also be facilely labeled with fluorescent AIEgens via this strategy. Herein, metal-free bioconjugation based on activated alkynes not only provides a general platform for facile conjugation and labeling, but also a general methodology for materials modification, from organic/inorganic targets to living organisms, thus exhibiting broad appeal to material science, chemistry, and biology.